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<h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">Touchdown-PCR</span></h1>
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<p>Die <b>Touchdown-PCR</b> ist eine Variante der <a href="Polymerasekettenreaktion" class="mw-redirect" title="Polymerasekettenreaktion">Polymerasekettenreaktion</a>, bei der in jedem Zyklus der <a href="Amplifikation_(Genetik)" title="Amplifikation (Genetik)">Amplifikation</a> die <a href="Primerhybridisierung" title="Primerhybridisierung">Hybridisierungstemperatur</a> leicht abgesenkt wird, um in Mischungen spezifischere Bindungen zwischen Vorlage und <a href="Primer" title="Primer">Primern</a> zu erzielen.<sup id="cite_ref-PMID1861999_1-0" class="reference"><a href="#cite_note-PMID1861999-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup>
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<div class="mw-heading mw-heading2"><h2 id="Eigenschaften">Eigenschaften</h2></div>
<p>Bei der Touchdown-PCR wird im ersten Zyklus die maximale Hybridisierungstemperatur verwendet.<sup id="cite_ref-PMID8679209_2-0" class="reference"><a href="#cite_note-PMID8679209-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup> In jedem weiteren Zyklus wird sie um ein bis zwei Grad Celsius abgesenkt. Sie wird eingesetzt, wenn mehrere Primer oder mehrere Bindungsstellen für Primer und dementsprechend mehrere Hybridisierungstemperaturen vorkommen, wie z. B. degenerierte Primermischungen.<sup id="cite_ref-PMID8068332_3-0" class="reference"><a href="#cite_note-PMID8068332-3"><span class="cite-bracket">[</span>3<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-PMID39955471_4-0" class="reference"><a href="#cite_note-PMID39955471-4"><span class="cite-bracket">[</span>4<span class="cite-bracket">]</span></a></sup>
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<div class="mw-heading mw-heading2"><h2 id="Einzelnachweise">Einzelnachweise</h2></div>
<ol class="references">
<li id="cite_note-PMID1861999-1"><span class="mw-cite-backlink"><a href="#cite_ref-PMID1861999_1-0">↑</a></span> <span class="reference-text">R. H. Don, P. T. Cox, B. J. Wainwright, K. Baker, J. S. Mattick: '<i>Touchdown' PCR to circumvent spurious priming during gene amplification.</i> In: <i>Nucleic acids research.</i> Band 19, Nummer 14, Juli 1991, S. 4008, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1093/nar%2F19.14.4008">10.1093/nar/19.14.4008</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/1861999?dopt=Abstract">PMID 1861999</a>, <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC328507/">PMC 328507</a> (freier Volltext).</span>
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<li id="cite_note-PMID8679209-2"><span class="mw-cite-backlink"><a href="#cite_ref-PMID8679209_2-0">↑</a></span> <span class="reference-text">K. H. Hecker, K. H. Roux: <i>High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.</i> In: <i>BioTechniques.</i> Band 20, Nummer 3, März 1996, S. 478–485, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.2144/19962003478">10.2144/19962003478</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/8679209?dopt=Abstract">PMID 8679209</a>.</span>
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<li id="cite_note-PMID8068332-3"><span class="mw-cite-backlink"><a href="#cite_ref-PMID8068332_3-0">↑</a></span> <span class="reference-text">K. H. Roux: <i>Using mismatched primer-template pairs in touchdown PCR.</i> In: <i>BioTechniques.</i> Band 16, Nummer 5, Mai 1994, S. 812–814, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/8068332?dopt=Abstract">PMID 8068332</a>.</span>
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<li id="cite_note-PMID39955471-4"><span class="mw-cite-backlink"><a href="#cite_ref-PMID39955471_4-0">↑</a></span> <span class="reference-text">S. M. Hussain, A. Sharif, F. Bashir, S. Ali, A. Javid, A. I. Hussain, A. Ghafoor, M. A. Alshehri, A. Naeem, E. Naeem, M. Amjad: <i>Polymerase Chain Reaction: A Toolbox for Molecular Discovery.</i> In: <i>Molecular Biotechnology.</i> [elektronische Veröffentlichung vor dem Druck] Februar 2025, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1007/s12033-025-01390-z">10.1007/s12033-025-01390-z</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/39955471?dopt=Abstract">PMID 39955471</a> (Review).</span>
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